model：jx-cksm-6wk
brand：shanghai jingxin
specifications ：pcs
introduction：single cell preparation instrument (6 channel heating type) is a new generation of cell suspension preparation equipment that integrates metal bath digestion and gentle tissue shearing.
product description:
the single cell preparation instrument is a new generation of cell suspension preparation equipment that integrates metal bath digestion and gentle tissue shearing. it is used in experiments such as flow cytometry/single cell sequencing/primary cell culture. it has a small amount of starting tissue. the time is short, the cell yield is high, and the cell activity is high. designed by the international din method, low noise, easy to use, stable performance, with heating function, the digestion process of the sample is always in a constant temperature state.
working principle:
the sample is mixed with high-density zirconia shearing magnetic beads and digestive enzymes, and then digested at a constant temperature and the tissue is sheared to obtain a high-concentration living cell suspension. subsequent steps such as digestion termination, filtration and washing, and red blood cell lysis if necessary, can obtain tens of millions to millions of live cells, and different tissues can reach a cell yield of more than 90%.
sample range
application areas: cancer research, cardiovascular research, stem cell research, immune research, neuroscience research
applications:
single-cell rnaseq
multicolor flow cytometry
mass cytometry
primary cell isolation and culture
car-t
steps:
advantage：
high tissue utilization, high efficiency of single cell process, high output of single cell
for clinical puncture samples, the tissue utilization rate can reach 100%, and the cell yield can reach more than 100,000
for the original cell culture sample, the process from tissue to single cell is completed within 15 minutes, reducing the time of cell adversity and improving cell survival rate
sequencing for tissue single cells, rapid single-cell processing, and high-yield single cells with a viability of over 85%.
take mouse tissue as an example:
technical parameter:
the main parameters | parameter range |
adjustable temperature: | 22℃-56℃ |
high-throughput processing samples: | process 6 2ml centrifuge tubes at the same time |
easy to use: | the preparation process is fully automatic, and a large amount of single cell suspension can be obtained in only 30 minutes |
high cell activity: | cell viability can reach more than 85% |
stable repeatability: | the same preparation procedure obtains the same approximate effect |
program: | 6 groups of preset programs (upgradeable) |
plow noise: | ＜50db, use brushless motor |
security settings: | no wearing parts design, electromagnetic lock protection, stainless steel cavity, effective antibacterial / all aluminum alloy cnc cavity, durable |
sample size: | 5-1000mg |
operating time | about 10 mins |
speed: | 0-4000rpm |
working time: | 20-300min |
